Descripción
Registros
Los datos en este recurso de evento de muestreo han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 9 registros.
también existen 3 tablas de datos de extensiones. Un registro en una extensión provee información adicional sobre un registro en el core. El número de registros en cada tabla de datos de la extensión se ilustra a continuación.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Nordlund L M, Lüskow F, Lawrence E (2026). Invertebrate eDNA Gotland Summer 2021. Version 2.1. OBIS Secretariat. Samplingevent dataset. https://doi.org/10.25607/bgjoog
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es OBIS Secretariat. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento-NoComercial (CC-BY-NC 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: afdf89d6-4aa9-41e1-bc3d-cee33befa66c. OBIS Secretariat publica este recurso y está registrado en GBIF como un publicador de datos avalado por Ocean Biodiversity Information System.
Palabras clave
Samplingevent; Benthic invertebrates: http://vocab.nerc.ac.uk/collection/EXV/current/EXV068/ Zooplankton: http://vocab.nerc.ac.uk/collection/EXV/current/EXV057/
Contactos
- Originador ●
- Punto De Contacto
- Originador
https://orcid.org/https://orcid.org/0000-0002-2100-7012
- Originador
Cobertura geográfica
Coastal waters of Gotland in the central Baltic Sea
| Coordenadas límite | Latitud Mínima Longitud Mínima [56,253, 16,831], Latitud Máxima Longitud Máxima [58,654, 20,786] |
|---|
Cobertura temporal
| Fecha Inicial / Fecha Final | 2021-07-27 / 2021-09-08 |
|---|
Datos del proyecto
No hay descripción disponible
| Título | Biodiversity Survey Gotland Summer 2021 |
|---|
Personas asociadas al proyecto:
Métodos de muestreo
Environmental DNA (eDNA) water samples were collected using a stratified random sampling approach to ensure coverage across distinct areas of the coastal bays. At each of the three sites, three separate 5-litre water samples were collected from a depth of < 0.5 m below the surface, either from a boat or by snorkelling. Samples were immediately stored on ice in cooler boxes for transport. In the laboratory, 500 ml from each 5-litre collection was filtered through a Nalgene® analytical filter funnel with a 0.45 µm pore size. The resulting filters were preserved in 200-proof ethanol and stored at -20°C until further processing. At the time of collection, in situ measurements for temperature, salinity, and dissolved oxygen were recorded using a handheld Elma 795 Multi Chemistry instrument.
| Área de Estudio | This study characterises the coastal brackish-water invertebrate diversity around the island of Gotland, Sweden, in the central Baltic Sea. Sampling was conducted during the summer of 2021 (July–September) at three distinct coastal sites: Kappelshamnsviken (northwest coast), Lergrav, and Valleviken (northeast coast). The study focussed on shallow coastal margins where bottom depths are generally less than 5 m. The environmental setting of the study area is characterised by relatively stable, low-to-intermediate salinity (range 4.2–6.7) and varying sub-surface temperatures (range 12.1–19.7°C). The taxonomic scope covers 42 marine invertebrate taxa across eight animal phyla, including both pelagic (holoplanktonic and meroplanktonic) and benthic assemblages. |
|---|
Descripción de la metodología paso a paso:
- The analytical workflow followed a standardised marine eDNA metabarcoding pipeline: - DNA extraction & purification: DNA was extracted from the filters using a commercial kit with a modified protocol to maximise yields and purified to remove PCR inhibitors - PCR amplification: the V4–V5 region of the 18S rRNA gene was targeted to capture a broad range of invertebrates. Standard analysis included three PCR replicates per sample - Library preparation & sequencing: PCR amplicons were purified, quantified using a Qubit broad-range kit, and pooled into a final library at equal concentrations; sequencing was performed on an Illumina MiSeq V3 kit (10.5 pM) with a 20% PhiX spike-in - Bioinformatics & taxonomic assignment: Sequences were processed through a custom pipeline for quality filtering and Operational Taxonomic Unit (OTU) clustering. Taxonomic assignments were made by comparing consensus sequences against the NCBI nt and SILVA 18S (v138.1) databases, using the GBIF taxonomic backbone for consistency; minimum similarity thresholds were set at 98% for species, 95% for genus, and 92% for higher taxonomic levels - Data filtering & analysis: The final dataset was filtered to remove low-abundance OTUs (< 0.025% or < 10 reads) and common contaminants; for multivariate analysis, OTU data were transformed into presence–absence data to account for biological and methodological biases in read proportions
Metadatos adicionales
| Identificadores alternativos | 10.25607/bgjoog |
|---|---|
| afdf89d6-4aa9-41e1-bc3d-cee33befa66c | |
| https://ipt.obis.org/bioecoocean/resource?r=biodiversity_survey_gotland_summer_2021 |